When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Microplate Reader Shelf. 6) and 0. 3. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. VersaMax reads 96-well and strip-well plates. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). mums. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Stat Fax 4700 Microstrip Reader. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Wound healing assay. edu Contact Phone: 216-368-8962. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Samples with OD values of less than 0. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 304 signal level saturation. 1 in stock! Molecular Devices SpectraMax M2 Multilabel Microplate Reader. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Microplate Readers. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. SpectraMAX 340 microtiter plate reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Service parts for these models are not being produced and are no longer in stock. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. the SpectraMax iD3 is a fantastic plate reader for a. Gerritsma,B. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Cytotoxicity was determined by measuring the release of LDH. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). The absorbances were being measured at 515 nm. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. reader (SpectraMax 340; Molecular devices, LLc). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The first time you replace an OEM lamp you must use the N-34-MLA. Immunofluorescence and Aggregation Analysis. Donor filter: 616/10 nm. 5% phosphoric acid) in microplates at room temperature for 10 min. 7. Novak, Nathan J. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. Temperature Range: Ambient + 4 °C up to 45 °C. Labsystems Luminoskan RT Microplate Reader. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). FACS analysis. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Fig. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. SpectraMax 190 reads 96-well and strip-well plates. Description 1. Microscopy. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Supplier: Molecular Devices. gp70 blocking. Tunable for filterless flexibility. Integration delay. edu Contact Phone: 216-368-8962. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Donor filter: 620 nm. 5), L-ascorbic acid (25. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. well, and strip-well microplates. Call 1-800-745-2710. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. TGFα release was calculated as described in the Results. Immunofluorescence and Aggregation Analysis. Molecular Devices SPECTRAmax. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 340 nm. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . more on but is otherwise untested and sold AS-IS. Or scan up to 384 wells at any time to determine the best assay conditions. 0200-6117. Dissolve 13. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Description 1. Male 8 pin mini Din to Female 9D. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Spectrophotometer (SpectraMax 340). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 2. 306 plate air cal fail,low light. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Please see photos. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 20. , Sunnyvale, CA). 5. Molecular Device M5e. Quick View. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Molecular Devices SpectraMax Gemini XS Microplate Reader. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. Isolation of RNA and RT-PCR. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. In vivo anti-malarial assay in mice. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Plates were washed with 1× KP solution and blocked with. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Cell proliferation was measured using the MTT method. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Details. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. . SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. They are for reference only. 314 low lamp level warning. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Details. 4. , Sunnyvale, CA). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. A 0. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. control or celatrol-treated cells. Operators can conveniently select up to 6 wavelengths at a time for. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1 0 100 80 60 40 20 0 4. 0, MSD) or Spectramax 340 (Molecular Devices). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 81. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ) at 490 nm. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. Italic and boldface type are used for emphasis. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Exhibit with us {{>currencyLabel}} Back. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. chen@case. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Awareness Technology, Inc. Homeostatic model assessment for insulin. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . 30. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 2. Call 1-800-745-2710. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Alves, Amelia K. Heme was preincubated for 5 min with A when added before the metals. Page: 1/6. This was followed by an additional incubation at 37 °C for 30–60 min. Binding of capture antibodies to polystyrene beads and biotinylation. Assay setup for a 384-well low-volume plate. Percent viability was calculated by comparison with the control group. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. Includes: SpectraMax 340PC384. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. The Catalina. The plates were incubated for 4 additional hours at 37°C. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. 340 nm / 70 nm. This test was performed in duplicates. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. To. Values were determined according to a standard curve (log/log curve-fit). 306 plate air cal. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. MMP‑1 inhibition assay. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. The plate was covered and incubated for one hour at room temperature. 94 g calcium chloride dihydrate (both. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. (1988) and recorded at 412 nm. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Molecular Devices’ PathCheck® sensor technology. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Analysis of ALA by Ehrlich’s Reagent Assay. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. The SpectraMax® iD3 Multi Mode Microplate . 1. SpectraMax 384 Plus. 3: The cuvette chamber. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 314 low lamp level warning. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax Gemini EM & FLEXstation (bottom read) 1. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. SpectraMAX 340. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. 5 mL sample or standard solution was well mixed with 0. Fibrin clots were formed as described above with some modifications 46. reader (Molecular Devi ces). 5 ng/ml; estradiol: 10 pg/ml. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Figure 1. Microplate Readers. MCE has not independently confirmed the accuracy of these methods. 2. Cell permeabilization. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. TDP-43 stress granular cells were treated with 70% ice-cold. SoftMax Pro. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). G. 2. Read one sample or up to 384. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. All data were normalized to experimental day 0. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). 5. Figure 1. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Also for: Spectramax 190, Spectramax 340pc384, Versamax. 5. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. Quick View. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Vessel Sprout Formation Assay. 6) and 0. The absorbances were transformed to velocity of substrate consumption (nmol/min). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Statistical analysis. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Filterless readers provide consistent results with steady temperature regulation. Molecular Devices SpectraMax M5e Microplate Reader. 5), L-ascorbic acid (25. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. 5mM), and changes in OD 405 nm. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Acceptor: 665nm. A. (1993). With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Create custom fluorescence assays without having to purchase expensive filter sets. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 4) was followed according to Vanacker et al. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Molecular Devices SpectraMax M5e Microplate Reader $26,500. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 305 reference level saturation. Amino Acid Analysis. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. TDP-43 stress granular cells were treated with 70% ice-cold. used. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 4) was followed according to Vanacker et al. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. , Sunnyvale, CA, USA) at a wavelength of 450 nm. ): 8. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . It includes both a lamp and a new lamp holder. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Details. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). 2. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). N-34-LB. using SpectraMax i3 (Molecular devices, San Jose, USA). The sensitivities of the hormones are Prolactin: 0. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Acceptor filter: 665/10 nm. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. These models can no longer be repaired. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. StakMax Microplate Handling Stacker. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The slope of the linear portion of the kinetic curve generated from each well was used to. Each experiment was performed in duplicate and repeated at. Other technologies such as imaging. 2 g/L solution; dilute 1:10 in HBSS. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software.